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trcn0000281950  (Addgene inc)


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    Structured Review

    Addgene inc trcn0000281950
    Trcn0000281950, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trcn0000281950/tet_pLKO%2E1_puro_shNRF2+%231+(Plasmid+%23136584)/pmc07315801-591-28-29
    Average 93 stars, based on 7 article reviews
    trcn0000281950 - by Bioz Stars, 2026-10
    93/100 stars

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    shRNA:

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. shRNA targeting sequences from the RNAi consortium ( 107 ) were cloned into tet-pLKO.1-puro as previously described ( 38 ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. shRNA targeting sequences from the RNAi consortium ( ) were cloned into tet-pLKO.1-puro as previously described ( ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. Plasmids shRNA targeting sequences from the RNAi consortium ( 107 ) were cloned into tet-pLKO.1-puro as previously described ( 38 ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.

    Clone Assay:

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. shRNA targeting sequences from the RNAi consortium ( 107 ) were cloned into tet-pLKO.1-puro as previously described ( 38 ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. shRNA targeting sequences from the RNAi consortium ( ) were cloned into tet-pLKO.1-puro as previously described ( ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.

    Article Title: Sporadic activation of an oxidative stress-dependent NRF2–p53 signaling network in breast epithelial spheroids and premalignancies
    Article Snippet: .. Plasmids shRNA targeting sequences from the RNAi consortium ( 107 ) were cloned into tet-pLKO.1-puro as previously described ( 38 ) for shLuc (TRCN0000072250, Addgene #136587), shNRF2 #1 (TRCN0000281950, Addgene #136584), shNRF2 #2 (TRCN0000284998, Addgene #136585), shJUND #1 (TRCN0000416347, Addgene #136581), shJUND #2 (TRCN0000416920, Addgene #136583). .. For the mRFP1-NRF2 reporter (Addgene #136580), the DNA binding domain of NRF2 was mutated (C506S) along with four leucines (L4A) in the leucine zipper region of the bZIP domain by site-directed mutagenesis of the pBabe mRFP1-NRF2 hygro plasmid (Addgene #136579) originally prepared by subcloning into pBabe mRFP1 hygro.



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    trcn0000281950  (Addgene inc)
    93
    Addgene inc trcn0000281950
    Trcn0000281950, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trcn0000281950/tet_pLKO%2E1_puro_shNRF2+%231+(Plasmid+%23136584)/pmc07315801-591-28-29
    Average 93 stars, based on 1 article reviews
    trcn0000281950 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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